Review




Structured Review

STEMCELL Technologies Inc rosettesep nk-cell enrichment mixture method
Rosettesep Nk Cell Enrichment Mixture Method, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosettesep+nk-cell+enrichment+mixture+method/rosettesep+human+b+cell+enrichment+cocktail/pmc10332273-65-11-16
Average 90 stars, based on 1 article reviews
rosettesep nk-cell enrichment mixture method - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Isolation:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Centrifugation:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Expressing:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Western Blot:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Two Tailed Test:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Transfection:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Plasmid Preparation:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Lysis:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Positive Control:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Release Assay:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Degranulation Assay:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Activity Assay:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Marker:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Immunohistochemistry:

Article Title: GD2 redirected CAR T and activated NK-cell-mediated secretion of IFNγ overcomes MYCN-dependent IDO1 inhibition, contributing to neuroblastoma cell immune escape
Article Snippet: Human NK cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Lympholyte Cedarlane) centrifugation.

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and Ficoll-Paque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.

Article Title: ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
Article Snippet: In some experiments, human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies) and Ficoll-Paque Plus (Amersham) centrifugation.

Article Title: AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation
Article Snippet: Human NK-cells were isolated from PBMC of healthy donors with the RosetteSep NK-cell enrichment mixture method (Stem-Cell Technologies, IT).

Article Title: The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors.
Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMCs) of healthy donors with the RosetteSep NK-cell enrichment mixture method (StemCell Technologies, Vancouver, Canada) and FicollPaque Plus (Lympholyte Cedarlane, Burlington, Ontario, Canada) centrifugation.



Similar Products

90
STEMCELL Technologies Inc rosettesep nk-cell enrichment mixture method
Rosettesep Nk Cell Enrichment Mixture Method, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosettesep+nk-cell+enrichment+mixture+method/rosettesep+human+b+cell+enrichment+cocktail/pmc10332273-65-11-16
Average 90 stars, based on 1 article reviews
rosettesep nk-cell enrichment mixture method - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc rosettesep nk-cell enrichment mixture method kit
Rosettesep Nk Cell Enrichment Mixture Method Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosettesep+nk-cell+enrichment+mixture+method/rosettesep+human+b+cell+enrichment+cocktail/pmc09367496-33-12-18
Average 90 stars, based on 1 article reviews
rosettesep nk-cell enrichment mixture method kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc rosettesep nk cell enrichment mixture method
Inhibition of ERAP1 enhances <t>NK</t> <t>cell</t> killing of 221-B*51:01 cells. (A) , Example of degranulation by human CD56 + <t>NK</t> <t>cells</t> from one healthy donor expressing KIR2DL1, KIR2DL3, and KIR3DL1 measured as CD107a cell surface expression in response to the indicated cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) Summary of NK cell degranulation from donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.
Rosettesep Nk Cell Enrichment Mixture Method, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosettesep+nk-cell+enrichment+mixture+method/rosettesep+human+b+cell+enrichment+cocktail/pmc08669763-57-22-28
Average 90 stars, based on 1 article reviews
rosettesep nk cell enrichment mixture method - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc rosettesep nk cell-enrichment mixture method
Inhibition of ERAP1 enhances <t>NK</t> <t>cell</t> killing of 221-B*51:01 cells. (A) , Example of degranulation by human CD56 + <t>NK</t> <t>cells</t> from one healthy donor expressing KIR2DL1, KIR2DL3, and KIR3DL1 measured as CD107a cell surface expression in response to the indicated cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) Summary of NK cell degranulation from donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.
Rosettesep Nk Cell Enrichment Mixture Method, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosettesep+nk-cell+enrichment+mixture+method/rosettesep+human+b+cell+enrichment+cocktail/pmc05901817-57-16-21
Average 90 stars, based on 1 article reviews
rosettesep nk cell-enrichment mixture method - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Inhibition of ERAP1 enhances NK cell killing of 221-B*51:01 cells. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor expressing KIR2DL1, KIR2DL3, and KIR3DL1 measured as CD107a cell surface expression in response to the indicated cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) Summary of NK cell degranulation from donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

Journal: Frontiers in Immunology

Article Title: ERAP1 Controls the Interaction of the Inhibitory Receptor KIR3DL1 With HLA-B51:01 by Affecting Natural Killer Cell Function

doi: 10.3389/fimmu.2021.778103

Figure Lengend Snippet: Inhibition of ERAP1 enhances NK cell killing of 221-B*51:01 cells. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor expressing KIR2DL1, KIR2DL3, and KIR3DL1 measured as CD107a cell surface expression in response to the indicated cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) Summary of NK cell degranulation from donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMC) of healthy donors co-expressing KIR2DL1, KIR2DL2 and KIR3DL1 by using the RosetteSep NK cell enrichment mixture method (Stem-Cell Technologies).

Techniques: Inhibition, Expressing, Transfection, Two Tailed Test

Inhibition of ERAP1 enhanced NK cell killing of 221-B*51:01 cells by affecting the engagement of KIR3DL1. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor co-expressing KIR2DL1, KIR2DL3, and KIR3DL1; CD107a cell surface expression was measured in specific KIR single positive (KIRsp) subsets (KIR3DL1sp for 221-B51 cells, KIR2DL1sp for 221-Cw4 cells and KIR2DL3 for 221-Cw3 and -Cw7 cells) in response to ERAP1-inhibited 221 transfectants and control cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) , Summary of NK cell degranulation in the indicated KIRsp NK subsets from healthy donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

Journal: Frontiers in Immunology

Article Title: ERAP1 Controls the Interaction of the Inhibitory Receptor KIR3DL1 With HLA-B51:01 by Affecting Natural Killer Cell Function

doi: 10.3389/fimmu.2021.778103

Figure Lengend Snippet: Inhibition of ERAP1 enhanced NK cell killing of 221-B*51:01 cells by affecting the engagement of KIR3DL1. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor co-expressing KIR2DL1, KIR2DL3, and KIR3DL1; CD107a cell surface expression was measured in specific KIR single positive (KIRsp) subsets (KIR3DL1sp for 221-B51 cells, KIR2DL1sp for 221-Cw4 cells and KIR2DL3 for 221-Cw3 and -Cw7 cells) in response to ERAP1-inhibited 221 transfectants and control cells. The percentage of CD107a + cells within the indicated NK cell subsets is shown. (B) , Summary of NK cell degranulation in the indicated KIRsp NK subsets from healthy donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMC) of healthy donors co-expressing KIR2DL1, KIR2DL2 and KIR3DL1 by using the RosetteSep NK cell enrichment mixture method (Stem-Cell Technologies).

Techniques: Inhibition, Expressing, Control, Transfection, Two Tailed Test

Inhibition of ERAP1 enhanced NK cell killing of 221-B*51:01 cells by affecting the engagement of NKG2A. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor; CD107a cell surface expression was measured in NKG2A positive subset negative for KIR3DL1, KIR2DL1 and KIR2DL3 in response to ERAP1-inhibited 221 transfectants and control cells. The percentage of CD107a + cells within the indicated NK cell subset is shown. (B) , Summary of NK cell degranulation in the NKG2A positive KIR negative NK subset from healthy donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

Journal: Frontiers in Immunology

Article Title: ERAP1 Controls the Interaction of the Inhibitory Receptor KIR3DL1 With HLA-B51:01 by Affecting Natural Killer Cell Function

doi: 10.3389/fimmu.2021.778103

Figure Lengend Snippet: Inhibition of ERAP1 enhanced NK cell killing of 221-B*51:01 cells by affecting the engagement of NKG2A. (A) , Example of degranulation by human CD56 + NK cells from one healthy donor; CD107a cell surface expression was measured in NKG2A positive subset negative for KIR3DL1, KIR2DL1 and KIR2DL3 in response to ERAP1-inhibited 221 transfectants and control cells. The percentage of CD107a + cells within the indicated NK cell subset is shown. (B) , Summary of NK cell degranulation in the NKG2A positive KIR negative NK subset from healthy donors after stimulation with 221 parental and transfectant cells in response to ERAP1 inhibition. P values, calculated by two-tailed paired Student’s t -test. ns, not significant.

Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMC) of healthy donors co-expressing KIR2DL1, KIR2DL2 and KIR3DL1 by using the RosetteSep NK cell enrichment mixture method (Stem-Cell Technologies).

Techniques: Inhibition, Expressing, Control, Transfection, Two Tailed Test

Genetical and pharmacological inhibition of ERAP1 makes 221-B*51:01 cells more susceptible to lysis by NK cells expressing KIR3DL1*001. (A, B) 221 transfectant cells genetically (A) or pharmacologically (B) inhibited for ERAP1 were incubated with YTS or YTS-KIR3DL1*001 NK cells. A representative experiment of three independently performed is shown. Data represented mean ± SD of triplicate.

Journal: Frontiers in Immunology

Article Title: ERAP1 Controls the Interaction of the Inhibitory Receptor KIR3DL1 With HLA-B51:01 by Affecting Natural Killer Cell Function

doi: 10.3389/fimmu.2021.778103

Figure Lengend Snippet: Genetical and pharmacological inhibition of ERAP1 makes 221-B*51:01 cells more susceptible to lysis by NK cells expressing KIR3DL1*001. (A, B) 221 transfectant cells genetically (A) or pharmacologically (B) inhibited for ERAP1 were incubated with YTS or YTS-KIR3DL1*001 NK cells. A representative experiment of three independently performed is shown. Data represented mean ± SD of triplicate.

Article Snippet: Human NK cells were isolated from peripheral blood mononuclear cells (PBMC) of healthy donors co-expressing KIR2DL1, KIR2DL2 and KIR3DL1 by using the RosetteSep NK cell enrichment mixture method (Stem-Cell Technologies).

Techniques: Inhibition, Lysis, Expressing, Transfection, Incubation